In this course we shall describe techniques for isolating and purifying cellular components, and for studying protein action in the context of metabolism. We shall go on to discuss the metabolism of carbohydrates and pyruvate, and of fatty acids.  At the same time, we shall consider energy requirements and generation in metabolism, and provide an introduction to the field of microbiology.

Throughout the course we shall emphasise the experimental techniques, the evidence and the unifying concepts behind our current understanding of metabolic processes.

By the end of this module you should be able to:

• describe some of the techniques used to isolate cellular components and to purify and characterise proteins;
• describe the principles and practices of column chromatography and electrophoresis, and how to monitor the progress of a purification procedure;
• demonstrate understanding of the features of metabolism exemplified by the major pathways of carbohydrate and fat metabolism (with introductory knowledge of amino acid metabolism);
• interpret disorders of metabolism by analysis of clinical data in case studies;
• show how specified major metabolic pathways can be analysed in terms of mechanisms and thermodynamics, and be able to outline the relevance of the state-of-the-art method of metabolomics.
• demonstrate observational, manipulative, numerical and deductive skills, and write reports (through experience gained in the practical and data-handling classes)
• develop improved skills of independent learning and verbal reporting in the clinical case studies
• develop knowledge of core microbiology including how to culture microorganisms